Scientific Research


Iris Run for Refugees - February 2010

Current research interests of the laboratory include the elucidation of the growth factor-mediated transformation process of the enterochromaffin-like cell (ECL) in the Mastomys model of gastric neoplasia, as well as identification of molecular signatures that delineate gastrointestinal carcinoids. A multidisciplinary team has been established with collaborators from the Departments of Pathology and Mathematics, the HHMI Keck Institute (Affymetrix and Proteomics), and Medical Oncology at Yale University as well as with investigators in Norway, Germany, the United Kingdom and Israel. Established methodologies currently utilized include neuroendocrine cell isolation and enrichment, cell culture and in vitro analysis, Affymetrix gene profiling of rodent and human samples, real-time PCR, tissue microarray construction and analysis, and biostatistical data evaluation.

 

 

Mark Kidd, PhD

Laboratory Director

Major interests include the molecular pathobiology of gastric and small intestinal carcinoids. Principal focus is the characterization of molecular signatures that define malignancy.


Terrain projection showing a clear separation in transcriptome profiles of Normal Tissue (n=4) and Neuroendocrine tumors (n=9)
 

 

 

Recent Publications or Manuscripts in press

 

1.      Kidd M, Siddique Z-L, Drozdov I, Gustafsson BI, Camp RL, Black JW, Boyce M, Modlin IM. The CCK2 receptor antagonist, YF476, inhibits Mastomys ECL cell hyperplasia and gastric carcinoid tumor development. Regulatory Peptides (2010: accepted).

2.      Svejda B, Kidd M, Giovinazzo F, Eltawil K, Gustafsson BI, Pfragner R, Modlin IM. The 5-HT2B receptor plays a key regulatory role in both neuroendocrine tumor cell proliferation and the modulation of the fibroblast component of the neoplastic microenvironment. Cancer (2010: in press).

3.      Risch HA, Yu H, Lu L, Kidd M. ABO Blood Group and the Association Between Helicobacter pylori Seropositivity and Risk of Pancreas Cancer. J Natl Cancer Institute (2010: in press).

4.      Drozdov I, Kidd M, Gustafsson BI, Joseph R, Pfragner R, Modlin IM. Auto-regulatory Effects of Serotonin on the Proliferation and Associated Signaling Pathway of Lung and Small Intestine Neuroendocrine Cell Lines Cancer 2009;115:4934-45.

5.      Kidd M, Hauso , Drozdov I, Gustafsson BI, Modlin IM. Delineation of the chemomechanosensory regulation of gastrin secretion using pure rodent G cells. Gastroenterology 2009; 137:231-41.

6.      Drozdov I, Kidd M, Nadler B, Camp R, Mane M, Gustafsson BI, Hauso O, Modlin IM. Predicting Enterochromaffin Cell Neoplasia Using Gene Expression Profiling and Supervised Machine Learning. Cancer 2009; 115:1638-50.

7.      Siddique Z-L, Drozdov I, Floch J, Gustafsson BI, Stunes K, Pfragner R, Kidd M, Modlin IM. KRJ-I and BON cell lines: defining an appropriate enterochromaffin cell neuroendocrine tumor model. Neuroendocrinology 2009; 89: 458-70.

8.      Modlin IM, Gustafsson BI, Drozdov I, Kidd M. Principal Component Analysis, Hierarchical Clustering, and Decision Tree Assessment of Plasma mRNA and Hormone Levels as an Early Detection Strategy for Small Intestinal Neuroendocrine (Carcinoid) Tumors. Annals Surgical Oncology 2009; 16: 487-98.

9.      Kidd M, Gustafsson BI, Drozdov I, Modlin IM. Serotonin secretion from Crohn’s disease EC cells is increased and mediated by IL1b and LPS through TOLL receptor 4 (TLR4) activation of NFκb. Neurogastroenterology & Motility 2009; 21: 439-50.

10.  Kidd M, Modlin IM, Gustafsson BI, Drozdov I, Hauso O, Pfragner R. Role of tastants and olfactants in the regulation of normal and neoplastic EC cell serotonin release. Am J Physiol 2008; 295:G260-72.

11.  Kidd M, Drozdov I, Joseph R, Pfragner R, Culler M, Modlin IM. Differential cytotoxicity of novel somatostatin and dopamine chimeric compounds on bronchopulmonary and small intestinal neuroendocrine tumor cell lines. Cancer 2008; 113:690-700.

12.  Bornschein J, Kidd M, Eick GN, Pfragner R, Modlin IM. Systematic Cytotoxic Analysis of Common Chemotherapeutic Agents On Lung and Small intestinal Carcinoid Cell lines. Journal of Cancer Molecules 2008; 4:47-54.

13.  Kidd M, Schally AV, Pfragner R, Malfertheiner MV, Modlin IM. Inhibition of Proliferation of Small Intestinal and Bronchopulmonary cell lines using analogues targeting receptors. Cancer 2008; 112:1404-14.

14.  Kidd M, Nadler B, Mane SM, Eick GN, Champaneria MC, Pfragner R, Modlin IM. GeneChip, geNorm and GI carcinoids: novel reference genes for real-time PCR. Physiological Genomics 2007; 30: 363-70.

15.  Kidd M, Modlin IM, Pfragner R, Eick GN, Champaneria MC, Chan AO, Camp RL, Mane SM. Small bowel Carcinoid (EC cell) Neoplasia exhibits TGFb1-Mediated regulatory abnormalities including Up-regulation of C-Myc and MTA1 Cancer 2007; 109: 2420-31.

16.  Kidd M, Modlin IM, Shapiro MD, Camp RL, Mane SM, Lye KD, Usinger W, Murren J. CTGF, intestinal stellate cells and carcinoid tumor fibrogenesis. World Journal of Gastroenterology 2007; 13:5208-16.

17.  Kidd M, Modlin IM, Black JW, Boyce M, Culler M. Examination of novel gastrin, somatostatin and dopamine receptor compounds on gastric enterochromaffin-like cell function. Regulatory Peptides 2007; 143:109-17.

18.  Kidd M, Eick GN, Mane SM, Nadler B, Champaneria MC, Pfragner R, Modlin IM. Characterization of a human neoplastic enterochromaffin (EC) cell line, KRJ-I, and the inhibitory effects of Lanreotide and Rapamycin. J Molecular Endocrinology 2007; 38:181-92.

19.  Kidd M, Modlin IM, Eick GN, Camp RL, Mane SM. The role of CCN2/CTGF in the proliferation of Mastomys Enterochromaffin-like cells and gastric carcinoid development. Am J Physiol 2007; 292:G191-200.

20.  Kidd M, Modlin IM, Eick GN, Champaneria MC. Isolation, Functional Characterization and Transcriptome of Mastomys Ileal Enterochromaffin Cells. Am J Physiol 2006; 291:G778-91.

21.  Modlin IM, Kidd M, Latich I, Zikusoka MN, Eick G, Mane SM, Camp RL. Genetic differentiation of appendiceal carcinoid malignancy – a guide for the perplexed. Annals of Surgery 2006; 244:52-60.

22.  Modlin IM, Kidd M, Pfragner R, Eick GN, Champaneria MC. The Functional Characterization of Normal and Neoplastic Human Enterochromaffin Cells. J Clinical Endocrinology and Metabolism 2006; 91:2340-8.

23.  Kidd M, Modlin IM, Mane SM, Camp RL, Eick G, Latic I, Zikusoka MN. The utility of molecular genetic signatures in the delineation of gastric neoplasia. Cancer 2006; 106:1480-88.

24.  Kidd M, Modlin IM, Mane SM, Camp RL, Eick G, Latich I. The role of genetic markers, NAP1L1, MAGE-D2 and MTA1, in defining small intestinal carcinoid neoplasia. Annals of Surgical Oncology 2006; 13:253-62.

25.  Kidd M, Modlin IM, Mane SM, Camp RL, Shapiro MD. Q RT-PCR detection of Chromogranin A: A new standard in the identification of neuroendocrine tumor disease. Annals of Surgery 2006; 204: 273-80.

26.  Zikusoka MN, Kidd M, Eick G, Latich I, Modlin IM. Molecular genetics of gastroenteropancreatic neuroendocrine tumors. Cancer 2005; 104:2292-309.

27.  Kidd M, Eick G, Shapiro MD, Camp RL, Mane SM, Modlin IM. Microsatellite instability and gene mutations in TGFbRII are absent in small bowel carcinoids. Cancer 2005; 103: 229-36.

 

 

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